Composite

Part:BBa_K4201020

Designed by: David Birkhaeuser   Group: iGEM22_CU-Boulder   (2022-10-12)


CrtE_cytoTDS2__T5αOH_RUBY

This construct is made of four separate segments: CrtE, cytoTDS2, T5αOH, and RUBY. Each segment is enclosed by an upstream Gmubi promoter and downstream AtHSP terminator. CrtE encodes for a GGPP synthase that converts DMAPP into GGPP1, a precursor in the paclitaxel pathway. Taxadiene synthase II, an enzyme encoded by cytoTDS2, then converts the GGPP into taxadiene2. This taxadiene is used by T5αOH to synthesize Taxadiene-5α-ol, the next molecule in the paclitaxel pathway3.

RUBY is a genetic reporter that helps visualizing genetic transformation by turning the affected plant a purple-red color4.


The construct is made to induce the biosynthesis of taxadiene, and consequently Taxadiene-5α-ol, in Glycine max.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6848
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 8395
    Illegal PstI site found at 13603
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6848
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 8395
    Illegal PstI site found at 13603
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6848
    Illegal BglII site found at 790
    Illegal BglII site found at 1153
    Illegal BglII site found at 2021
    Illegal BglII site found at 2956
    Illegal BglII site found at 4011
    Illegal BglII site found at 5324
    Illegal BglII site found at 5572
    Illegal BamHI site found at 11815
    Illegal XhoI site found at 3601
    Illegal XhoI site found at 3868
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6848
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 8395
    Illegal PstI site found at 13603
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6848
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 8395
    Illegal PstI site found at 13603
    Illegal NgoMIV site found at 1697
    Illegal NgoMIV site found at 6997
    Illegal NgoMIV site found at 9751
    Illegal NgoMIV site found at 10330
    Illegal NgoMIV site found at 12043
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 948
    Illegal BsaI site found at 1887
    Illegal BsaI site found at 2167
    Illegal BsaI site found at 3113
    Illegal BsaI site found at 5438
    Illegal BsaI site found at 5718
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 1873
    Illegal BsaI.rc site found at 2153
    Illegal BsaI.rc site found at 3100
    Illegal BsaI.rc site found at 5424
    Illegal BsaI.rc site found at 5704
    Illegal BsaI.rc site found at 6651


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